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Midori Green Xtra is a new highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels. This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with Midori Green Xtra have a very low background fluorescence, which makes.
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MIDORI Green Direct resulted in a dramatic increase of positive transformants. Midori Green Can Boost Your Cloning Results! Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short-wavelength light is known to cause thymine dimers and damage DNA.
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MIDORI Green Advance is a safe alternative to the traditional nucleic acid stain ethidium bromide. It is a non-carcinogenic and less mutagenic dye for detecting dsDNA, ssDNA and RNA in agarose gels with a very high sensitivity. MIDORI Green Advance can utilize with UV light or with our innovative Blue/Green LED technology.
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MIDORI Green Easy (MG12) comes in a volume of 0.4 mL and a concentration of 10,000 x (for staining 4 L of agarose). This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with MIDORI Green Easy have a very low background fluorescence, which makes the.
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MIDORI Green Direct resulted in a dramatic increase of positive transformants. Midori Green Can Boost Your Cloning Results! Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short-wavelength light is known to cause thymine dimers and damage DNA.
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In that respect, MIDORI Green Xtra is significantly superior to other products. Perfectly fit to FAS-Nano's Blue/Green LED, and the band was clearly visible. With this instrument, you can take an image with iPad and see the results immediately while giving an explanation of electrophoresis result to my students. Blue LED also worked with this.
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There are three reasons: 1) Midori Green binds less aggressively to DNA molecules, so fewer mutations occur during synthesis. 2) Midori Green does not penetrate the skin or plasma membranes, so uptake into living cells is quite minimal. 3) Midori Green has a very short half-life, so it breaks down in the environment quickly.
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Midori Green Direct should be used when high detection sensitivity is required, such as to detect small amounts of DNA fragments, whereas EZ-Vision can be implemented when DNA fragments are detected as part of the routine DNA work because EZ-Vision is the cheapest among non-mutagenic and loading dye-type DNA-staining reagents (Table 2).
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Midori Green Advance — Safe DNA Stain, a safe alternative to ethidium bromideReady-to-use electrophoresis buffers, gels, and powder stains quickly sort sample molecules based on size and charge. These reagents generate less background fluorescence that can negatively impact visual studies. To achieve the necessary chemical reaction, select the type of stain based on environmental conditions.
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MIDORI Green Advance is added into the agarose gel prior to running samples (much like EtBr), and provides sensitivity of detection on par with EtBr when visualized with blue LEDs or Nippon Genetics' superior Blue/Green LED technology. The second, MIDORI Green Direct, is designed to mix with your sample and load onto a stain-free gel.
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MIDORI Green Direct stain is non-carcinogenic and less mutagenic compared to ethidium bromide. Furthermore, we can state that MIDORI Green Direct is impenetrable to latex gloves and cell membranes. MIDORI Green Direct is classified as non-hazardous to aquatic life, under CCR Title 22 regulation. Thus, small amounts of MIDORI Green Direct stain.
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• For the labeling of nucleic acids • Non-carcinogenic alternative to ethidium bromide • Storage solution without DMSO 3 versions: • Midori Green Advance: high sensitivity for small fragments, background noise reduction, dye to be added during the preparation of the gel or following the migration, 4 - 6 µl for 100 ml of agarose; main excitation wavelength 490 nm / secondary 290 nm.
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Midori Green DNA Stain is a nucleic acid stain which can be used as a safer alternative to the traditional Ethidium Bro-mide stain for detecting nucleic acid in agarose gels. It is as sensitive as ethidium bromide and can be used exactly the same way in agarose gel electrophoresis (Figure 1).
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MIDORI Green Advance. MIDORI Green Advance is optimized for use with UV-light transilluminators. Compared to ethidium bromide (EtBr), MIDORI Green Advance is non-toxic and non-carcinogenic but delivers very strong signals of DNA/RNA with an excellent signal-to-background ratio. These properties will make it a perfect replacement for EtBr, if UV-light is used as the excitation source.